Effects of dipotassium trioxohydroxytetrafluorotriborate (K₂[B₃O₃F₄OH]) on genetic material and inhibition of cell division in human cell cultures

Abstract

We have examined antiproliferative, cytotoxic, and genotoxic potential of a halogenated boroxine dipotassium trioxohydroxytetrafluorotriborate [K₂(B₃O₃F₄OH)]. The impact on cell growth was evaluated by alamarBlue assay in basal cell carcinoma culture. Cytostatic, cytotoxic, and genotoxic potential were evaluated in lymphocytes culture, applying cytokinesis-block micronucleus cytome assay and chromosome aberrations analysis. Tested concentrations (0.05, 0.1, 0.2, and 0.4 mg/mL) were correlated with inhibition of cell growth in basal cell carcinoma culture and with the lymphocytes proliferation. Clastogenic activity has been confirmed, without evidences of aneugenic activity, in human lymphocytes.

Keywords::

• Boroxine
• chromosome aberrations
• micronuclei
• alamar blue
• basal cell carcinoma