Cofactor metals and antioxidant enzymes in cisplatin-treated rats: effect of antioxidant intervention

Abstract

We explored the association between the activities of antioxidant enzymes and their metallic cofactors in rats treated with cisplatin. The antioxidant effects of aminoguanidine, and a combination of vitamins E and C were investigated. Plasma platin was significantly lower than liver and kidney. Cisplatin treatment caused significant increase in plasma Se-glutathione peroxidase activity. Activities of Se-glutathione peroxidase, glutathione S-transferase, catalase and Cu,Zn-superoxide dismutase have been found to be significantly decreased in liver and kidney compared to controls. Zn levels in these organs were diminished upon cisplatin treatment, while levels of Cu were unaffected. Interestingly, levels of iron, the cofactor of catalase, were found to be significantly increased in liver and kidney. Intervention with aminoguanidine or vitamins was generally prevented cisplatin-caused changes in the activity of enzymes and in the tissue levels of cofactor metals. These observations suggest that relation between activities of enzymes and levels of cofactor metals is multifactorial.

• Antioxidant enzymes
• cisplatin
• in vivo
• metallic cofactors
• rat

Acknowledgements

Gozde Girgin and Melis Karaca were acknowledged for their technical assistance and help in data preparation, respectively.

Declaration of interest

The authors declare that there is no conflict of interest. This study was supported by Hacettepe University Research Center Office, Grant no. 04 DD 02 301 002.