The Effects of Ellagic Acid on Arylamine N-Acetyltransferase Activity in the Bacterium Pseudomonas Aeruginosa

ABSTRACT

Arylamine N-acetyltransferase (NAT) activity in Pseudomonas aerusinosa was inhibited by ellagic acid (EA), a naturally occurring dietary plant phenol. By measuring the acetylation of 2-aminofluorene (2-AF), the NAT activity was determined. In P. aerusinosa ATCC 27853, a NAT activity of 1.37± 0.25 nmol/min/IO¹⁰ CFU for intact cell and a NAT activity of 5.92 ± 0.20 nmol/min/mg protein for cytosolic preparation were measured. EA (ranging from 1 to 0.125 mM) showed a dose-dependent inhibition of NAT activities in the analysis of both intact cell and cytosolic preparations. Enzymatic kinetics were determined and found that EA was a potent non-competitive inhibitor of NAT activity in P. aerusinosa ATCC 27853. EA inhibition of NAT activities in P. aerusinosa ATCC 27853 was time-dependent for at least 4 hrs. These data strongly indicated that EA could suppress NAT activity in P. aerusinosa.