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Abstract
The enzymatic activities of lactate dehydrogenase (LDH) and α-hydroxybutyrate dehydrogenase (HBDH) were measured in 87 semen samples. The highest values of these enzymes correspond to the specimens with greatest sperm density and motility. There was a positive correlation between the enzymatic parameters and sperm density and motility. There was a positive correlation between the HBDH/LDH ratio and the percent LDH-X. The estimation of the HBDH/LDH ratio, easily and rapidly done, can be a substitute for direct LDH-X estimations. The HBDH/LDH ratio greater than 1.0 that was noted in men with good sperm motility and normal sperm counts, could represent a reliable index of semen quality.