Abstract
Adenylyl cyclase (AC) and protein carboxyl methylase (PCM) activities in human ejaculated spermatozoa were measured in 31 men being evaluated for infertility at the Infertility Clinic. The spermatozoal Mn2+ -sensitive AC activities ranging between zero and 1.4 nmol cyclic adenosine monophosphate (cAMP)/109 cells/min, was positively correlated with motility index [% motile sperm × degree (0–4)] (r = 0.72, p < 0.05). This confirms a role for cAMP in human sperm motility. PCM activities, ranging between zero and 160 pmoles (CH3OH/109 cells/min), were significantly correlated with AC activities (r = 0.86, p < 0.001). However, PCM activities were not positively correlated with motility index (p = 0.065). PCM most probably is a factor involved in spermatozoal motility since two patients suffering necrospermia possessed spermatozoa deficient in both AC and PCM activities.