Lack of Correlation Between Human y-Positive Spermatozoa and Acrosomal Abnormalities and Variations in Intensity of Fluorescence of Sperm Stained with Quinacrine Compounds

Abstract

Seminal fluid from 170 men was examined for acrosomal abnormalities of sperm (Papanicolaou procedure × 1000) and for y-positive cells (quinacrine-stained smears). No correlation was found between these parameters, suggesting that acrosomal abnormalities are similarly distributed among x- and y-bearing spermatozoa. The proportion of y-positive sperm was found to be low (23.6 + 10.7% (SD) for the oligozoospermic specimens and 23.7 ± 9.8% for specimens with sperm counts above 40 million/ml). Three degrees of fluorescence intensity were observed-weak, moderate, and strong-the strong fluorescence being associated with the highest percentage of y-bodies (30.6 ± 12.7%, 36.6 ± 5.7%) and the weak fluorescence with the lowest (16.7 ± 7.0%, 15.0 ± 9.5%). It is suggested that structural abnormalities and/or metabolic alterations in either the DNA molecule or the chromosomal proteins may be responsible for variability in the ability to bind quinacrine compounds.

Key Words:

• Human seminal fluid
• y-Positive spermatozoa
• y-Sperm-fluorescence intensity
• Acrosomal abnormalities.