Phosphorus-31 and Proton NMR Analysis of Reproductive Organs of Male Rats

Abstract

Phorphorus NMR spectra of the whole reproductive organs of male rats and their perchloric acid extracts indicate the presence of phosphocholine and phosphoethanolamine in the testis, glycerophosphocholine (GPC) and glycerophosphoethanolamine (GPE) in the epididymis, and creatine phosphate, GPC, and GPE in the seminal vesicles. High amounts of carnitine and inositol were observed by the proton NMR of perchloric acid extract of the corpus and cauda epididymis. Smaller amounts of these compounds were observed in the caput epididymis and vas deferens; they were totally absent in the testis. Creatine is present in high concentrations in the testis, vas deferens, and seminal vesicles. It is almost absent in all parts of the epididymis.

Key Words:

• Nuclear magnetic resonance
• P-31 NMR
• H-1 NMR
• Epididymis
• Seminal vesicles
• Testis