Structure of Human Sperm Chromatin: A Study on the Accessibility of DNA to Macromolecules

Abstract

The structure of human sperm chromatin compared with somatic chromatin (liver) was studied by titration of the exposed DNA-phosphate groups with poly-1-lysine (3000 and 28,100 MW) and by their susceptibility to the hydrolytic action of micrococcal nuclease and DNase I. With both sizes of polyly-sine used, the binding values were significantly lower for sperm chromatin (0.31 ± 0.05) than for liver chromatin (0.52 ± 0.05), indicating the presence of about 30% and 52% of free phosphate groups, respectively. Interaction with liver chromatin left no polylysine molecules partially unbound (“wastage”) even when 28,100 MW polylysine was used; on the contrary, sperm chromatin showed 26% of “wasted” polylysine even when the smaller polymer was used, indicating that in sperm chromatin the accessible DNA zones are usually no longer than 42 Å, that is, 12 base pair. Sperm chromatin was notably more susceptible to both micrococcal nuclease and DNase I action than liver chromatin. However, in the presence of saturating concentrations of polylysine they were similarly protected. Micrococcal nuclease and DNase I hydrolysis products of sperm fractions when submitted to electrophoresis produced a polydisperse smearing pattern along the gel that was difficult to correlate with the presence of nucleosomal structure.

Key Words:

• Sperm
• DNA
• Chromatin structure.