Publication Cover
Archives of Andrology
Journal of Reproductive Systems
Volume 25, 1990 - Issue 1
137
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Original Article

Cytogenetics of Porcine Sperm Chromosomes Following IVF of Zona-Free Hamster Oocytes

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Pages 45-57 | Received 31 Jan 1990, Published online: 09 Jul 2009
 

Abstract

Porcine sperm chromosome karyotypes were obtained from two boars following heterologous in vitro fertilization (IVF) of zona-free hamster ova with ionophore-capacitated porcine spermatozoa. The karyotype yield per inseminated ovum was 9.3% in = 1658), and there was no significant deviation from the expected 1 : 1 sex ratio (p > 0.5). Cytogenetic analysis revealed hyperhaploid (2.5%) and hypohaploid spermatozoa (1.9%). Spermatozoa with structural chromosome aberrations constituted 7.1% of the analyzed karyotypes, and these were predominantly chromosomal breaks, although acentric fragments were also found (2.6%).

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