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Abstract
Clinical investigations of the male partner of couples with recurrent miscarriage involves investigation of abnormalities in paternal chromosomes. This route of investigation does not include analysis of semen parameters. This investigation was conducted to evaluate DNA flow cytometry profiles of spermatozoa following staining with propidium iodide of couples attending the Pregnancy Loss Clinic for recurrent miscarriage. Ejaculates of 21 men were included in this study. DNA fluorescence was measured on the Becton Dickinson Fac Star Plus. Data were correlated with spermiogram parameters. The neat semen median minus peak channel values were negatively correlated with motility (p <. 05). Therefore, DNA flow cytometry can provide confirmatory evidence for conventional semen analysis and is useful as a preliminary screening procedure for clinical management of the couple. It could also be used in association with programs for the assessment of possible reproductive toxicity and infertility in couples with recurrent miscarriage.
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