Abstract
Total sperm count and percent sperm motility were noted in different parts of the epididymis, and the effect of anterior, middle, and posterior epididymal fluid on the induction of motility of nonmotile testicular spermatozoa was observed. The results were used to compare the influence of different parts of epididymis of Hemidactylus flaviviridison the acquisition of sperm motility, which is a sign of sperm maturation. Spermatozoa collected from different regions of the ductus epididymidis showed considerable difference in their motility as well as total count with an increase from anterior to posterior region. The epididymal fluids from different regions induced the motility of testicular spermatozoa and the induction accelerated with increasing fluid volume and incubation period. Epididymal fluid at higher volumes induced the motility of nonmotile testicular spermatozoa significantly even at short-term incubation. Among different epididymal regions, fluid from posterior was more potent in influencing the motility than the fluids of middle and anterior. Motility pattern of spermatozoa varied from zig-zag, circular, and erratic in anterior to wavy in posterior region of epididymis. However, the mean sperm velocity did not show any regional variation.
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