Techniques a Simple Method for the Collection of Cells from Cerebrospinal Fluid for Electron Microscopy

Louis Frans Department of Submicroscopic Morphology, University of Nijmegen Medical School, Geert Grooteplein Zuid 24, 6526, GA, Nijmegen, The Netherlands

Lutke Schipholt Department of Submicroscopic Morphology, University of Nijmegen Medical School, Geert Grooteplein Zuid 24, 6526, GA, Nijmegen, The Netherlands

Adrianus Martinus Stadhouders Department of Submicroscopic Morphology, University of Nijmegen Medical School, Geert Grooteplein Zuid 24, 6526, GA, Nijmegen, The Netherlands

Abstract

A simple membrane filter technique permits the collection of cells from cerebrospinal fluid for electron microscopy. The method is universally applicable for the concentration of small number of cells from free-floating cell suspensions and has several advantages: (1) Only a small fluid sample (1 ml) is needed. (2) Excellent cell preservation is obtainable. (3) High cell recovery rates are achieved. (4) Quantitative classification of cell types is possible.

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Techniques a Simple Method for the Collection of Cells from Cerebrospinal Fluid for Electron Microscopy

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Techniques a Simple Method for the Collection of Cells from Cerebrospinal Fluid for Electron Microscopy

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