Electron Microscopic and Immunoelectron Microscopic Demonstration of Pancreatic Polypeptide Cells in Glucagonoma: Colocalization of Pancreatic Peptide and Glucagon in Single Secretory Granules

Akishi Ooi Pathology Division, Department of Surgery, Cancer Research Institute HospitalKanazawa University, Kanazawa; Department of Pathology, School of Medicine, Kanazawa University, Kanazawa; Growth Factor Division, National Cancer Center Research Institute, Tokyo; Department of Surgery, Fukui General Hospital, Fukui; Department of Dermatology, Fujita Hospital, Fukui, Japan

Shogo Katsuda Pathology Division, Department of Surgery, Cancer Research Institute HospitalKanazawa University, Kanazawa; Department of Pathology, School of Medicine, Kanazawa University, Kanazawa; Growth Factor Division, National Cancer Center Research Institute, Tokyo; Department of Surgery, Fukui General Hospital, Fukui; Department of Dermatology, Fujita Hospital, Fukui, Japan

Isao Nakanishi Pathology Division, Department of Surgery, Cancer Research Institute HospitalKanazawa University, Kanazawa; Department of Pathology, School of Medicine, Kanazawa University, Kanazawa; Growth Factor Division, National Cancer Center Research Institute, Tokyo; Department of Surgery, Fukui General Hospital, Fukui; Department of Dermatology, Fujita Hospital, Fukui, Japan

Tbru Kameya Pathology Division, Department of Surgery, Cancer Research Institute HospitalKanazawa University, Kanazawa; Department of Pathology, School of Medicine, Kanazawa University, Kanazawa; Growth Factor Division, National Cancer Center Research Institute, Tokyo; Department of Surgery, Fukui General Hospital, Fukui; Department of Dermatology, Fujita Hospital, Fukui, Japan

Ken Aamaguchi Pathology Division, Department of Surgery, Cancer Research Institute HospitalKanazawa University, Kanazawa; Department of Pathology, School of Medicine, Kanazawa University, Kanazawa; Growth Factor Division, National Cancer Center Research Institute, Tokyo

Hideo Kitamura Pathology Division, Department of Surgery, Cancer Research Institute HospitalKanazawa University, Kanazawa; Department of Pathology, School of Medicine, Kanazawa University, Kanazawa; Growth Factor Division, National Cancer Center Research Institute, Tokyo; Department of Surgery, Fukui General Hospital, Fukui; Department of Dermatology, Fujita Hospital, Fukui, Japan

Yukinori Hayakawa Pathology Division, Department of Surgery, Cancer Research Institute HospitalKanazawa University, Kanazawa; Department of Pathology, School of Medicine, Kanazawa University, Kanazawa; Growth Factor Division, National Cancer Center Research Institute, Tokyo; Department of Surgery, Fukui General Hospital, Fukui; Department of Dermatology, Fujita Hospital, Fukui, Japan

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Abstract

We describe a case of pancreatic tumor in a 65-year-old woman with typical glucagonoma syndrome. Plasma glucagon (GL) and pancreatic polypeptide (PP) were markedly elevated up to 1404 and 1 200 pg/ml, respectively. Histologic examination of the metastatic tumors in liver and lymph nodes showed endocrine-type tumors composed of GL-positive cells some of which coexpressed PP immunoreactivity. Electron microscopy revealed the tumor cells with single-type secretory granules similar to normal A cell granules. Double immunogold staining demonstrated both GL and PP immuno-reactivities in the same secretory granules. Biologic and diagnostic significance of coexpression of PP and GL in a single secretory granules of pancreatic endocrine tumors is discussed briefly.

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Electron Microscopic and Immunoelectron Microscopic Demonstration of Pancreatic Polypeptide Cells in Glucagonoma: Colocalization of Pancreatic Peptide and Glucagon in Single Secretory Granules

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Electron Microscopic and Immunoelectron Microscopic Demonstration of Pancreatic Polypeptide Cells in Glucagonoma: Colocalization of Pancreatic Peptide and Glucagon in Single Secretory Granules

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