A supercritical fluid-based coating technology. 3: Preparation and characterization of bovine serum albumin particles coated with lipids

Abstract

Solvent-free microparticles, loaded with bovine serum albumin as a model protein, were produced using a novel supercritical (SC) fluid-based coating technology. Coating material consists either of trimyristin (Dynasan® 114) or of Gelucire® 50-02. Microparticles obtained were characterized as regards their morphology, protein content and in vitro release profile. A discontinuous coating made of micro-needles of trimyristin led to an initial burst release of ∼ 70% in 30min. However, a prolonged release of the BSA could be achieved in a phosphate buffer solution at 37 °C over a 24 h period from particles coated with Gelucire® 50-02. Furthermore, it was shown that BSA does not undergo any degradation after SC CO₂ treatment under the conditions used in the coating process.

Key Words:

• Supercritical carbon dioxide
• protein encapsulation
• protein release
• lipid coating
• capillary electrophoresis