Evaluation of toxicity and adjuvant effects of peptidoglycan microspheres orally administered to mice

Abstract

In this study, peptidoglycan microspheres were evaluated for their toxicity and adjuvant effects after oral administration to mice. The liver and spleen indexes, CD cell content in peripheral blood and spleen, and immunoglobulin content in peripheral blood were measured by flow cytometry and indirect ELISA, respectively. Peptidoglycan microspheres with a loading capacity of 46.41 ± 0.83 g/100 g were prepared. In vivo tests showed that peptidoglycan microspheres revealed an immuno-enhancing profile as indicated by the slow increase of IgG content in peripheral blood compared with that of the untreated peptidoglycan group. In conclusion, peptidoglycan microspheres may be used as a new oral adjuvant in the host.

• Adjuvant
• CD molecules
• immunoglobulinsm
• peptidoglycan microspheres
• sustained release

Declaration of interest

We gratefully acknowledge the support of the National Natural Science Foundation of China (No 31000773); Chinese Scholarship Committee (CSC); Open Item of Key Laboratory of Functional Inorganic Material Chemistry (Heilongjiang University), Ministry of Education and the Heilongjiang University Outstanding Youth Fund (201105).