Recombinant cDNA encapsulation in small liposomes with hepatocyte access ability

S. F. Aliño Department of Pharmacology, Faculty of Medicine and Density, University of Valencia, Avenida de Blasco Ibañ 15, 46010, Valencia, Spain

M. Bobadilla Department of Pharmacology, Faculty of Medicine and Density, University of Valencia, Avenida de Blasco Ibañ 15, 46010, Valencia, Spain

F. J. Unda Department of Cell Biology and Morphological Sciences, Faculty of Medicine and Dentistry, University of the Basque Country, Leioa, 48940, Vizcaya, Spain

M. Garcia-sanz Department of Pharmacology, Faculty of Medicine and Density, University of Valencia, Avenida de Blasco Ibañ 15, 46010, Valencia, Spain

E. Hilario Department of Cell Biology and Morphological Sciences, Faculty of Medicine and Dentistry, University of the Basque Country, Leioa, 48940, Vizcaya, Spain

M. Lejarreta Department of Pharmacology, Faculty of Medicine and Density, University of Valencia, Avenida de Blasco Ibañ 15, 46010, Valencia, Spain

Abstract

Liposomal encapsulation efficiency of a recombinant cDNA was studied by several procedures. We observed that supernatant fraction of ultracentrifuged liposomes prepared by extrusion through polycarbonate filters of 400 nm pore size yielded a very homogeneous suspension of small (50nm diameter) unilamellar liposomes with highest DNA/lipid ratio and great ability to access to hepatocytes.

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Recombinant cDNA encapsulation in small liposomes with hepatocyte access ability

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Recombinant cDNA encapsulation in small liposomes with hepatocyte access ability

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