Micro encapsulation of human insulin DEAE-dextran complex and the complex in liposomes by the emulsion non-solvent addition method

Abstract

Human insulin-DEAE (diethyl amino ethyl) dextran complex and human insulin DEAE-dextran complex in liposomes were encapsulated in cellulose acetate butyrate (CAB) microcapsules by the emulsion non-solvent addition method. The ratio of core-to-coat used was 1:1. The average diameters of the complex microcapsules and the complex liposome microcapsules were 239 ± 77 and 182 ± 52. m respectively. In vitro dissolution studies of both types of microcapsules in simulated intestinal fluid at pH 7 showed a sustained release of the complex and the complex liposome microcapsules with 0 = 1 h and respectively. This study can be applied to the further development of oral formulations of human insulin liposomes for diabetic treatment.

Key Words:

• Micro encapsulation
• insulin
• liposome
• emulsion
• non-solvent addi tion
• cellulose acetate butyrate