Characterization of ciprofloxacin liposomes: Derivative ultraviolet spectrophotometric determinations

Abstract

Neutral, (-) and (+) charged CF encapsulated liposome formulations prepared with EPC:Chol and DPPC:Chol lipids were investigated for their loading capacity, encapsulation % and release properties. CF amounts in the liposomes were estimated using derivative UV spectroscopy. Among the liposome formulations DPPC:Chol and EPC:Chol neutral formulations had a signifi cantly higher loading capacity and slowest release rate compared with (-) and (+) charged liposomes. The derivative UV spectroscopy was found to be an easy and sensitive method for direct estimation of CF in liposomes.

Key Words:

• Ciprofloxacin
• liposome entrapment
• drug release
• derivative spec-trophotometry