10
Views
76
CrossRef citations to date
0
Altmetric
Original Article

Evidence for two states of thermotolerance in mammalian cells

Pages 513-526 | Received 18 May 1987, Accepted 19 Nov 1987, Published online: 09 Jul 2009
 

Abstract

The effect of the inhibition of protein synthesis on the development of thermotolerance in Chinese hamster fibroblasts following a brief heat shock or exposure to sodium arsenite has been examined. Under conditions that inhibit protein synthesis by 95 per cent, significant amounts of thermotolerance develop after a brief exposure to 45°C or continuous exposure to 41°C, without the significant accumulation of heat shock proteins. However, no thermotolerance development in cells treated with sodium arsenite was observed if protein synthesis was inhibited. Heated cells which developed thermotolerance in the absence of protein synthesis are subject to the thermal sensitizing action of a subsequent exposure to amino acid analogues, while cells which developed thermotolerance with unimpeded protein synthesis are refractory. These results suggest that heat can simultaneously induce two states of thermotolerance, only one of which is dependent on protein synthesis. These two states can be distinguished operationally with respect to their response to amino acid analogue exposure.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.