Effects of verapamil and diltiazem on hyperthermic cell death in CHO cells

Ronald A. Coss Laboratory of Experimental Radiation Oncology, Department of Radiation Oncology and Nuclear Medicine, Thomas Jefferson University Hospital, Philadelphia, PA, 19107, USA

Raymond E. Felgar Laboratory of Experimental Radiation Oncology, Department of Radiation Oncology and Nuclear Medicine, Thomas Jefferson University Hospital, Philadelphia, PA, 19107, USA

David G. Stokes Laboratory of Experimental Radiation Oncology, Department of Radiation Oncology and Nuclear Medicine, Thomas Jefferson University Hospital, Philadelphia, PA, 19107, USA

Nancy N. Smith Laboratory of Experimental Radiation Oncology, Department of Radiation Oncology and Nuclear Medicine, Thomas Jefferson University Hospital, Philadelphia, PA, 19107, USA

Abstract

Non-toxic concentrations of verapamil (0.05 and 0.075 mM) and diltiazem (0.10 and 0.25 mM) sensitize CHO cells to heat killing at 44°C. These drugs sensitize by reducing both the shoulder and the slope of the exponential survival curve. Exposure to verapamil or diltiazem in conjunction with the heat sensitizer procaine HCl at 44°C reduced cell survival below that observed for procaine plus heat. The mechanism by which verapamil and diltiazem sensitize CHO cells to heat killing is not understood.

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Effects of verapamil and diltiazem on hyperthermic cell death in CHO cells

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Effects of verapamil and diltiazem on hyperthermic cell death in CHO cells

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