Variation in heat sensitivity through the cell cycle of M10 and Burkitt P3HR-1 cells

Abstract

The cell-cycle age response to 44 or 45°C hyperthermic treatment was studied in M10, a mutant of mouse L5178Y cells, and human Burkitt P3HR-1 cells synchronized by centrifugal elutriation. Survival response to 44°C hyperthermia or radiation showed that Burkitt cells were relatively hyperthermic resistant and average in radiosensitivity, while M10 cells were relatively hyperthermic sensitive and substantially radiosensitive. A typical age-response to hyperthermia through the cell-cycle was observed in M10 cells, although no significant variation in the response to hyperthermia was demonstrated in Burkitt P3HR-1 cells. Combined effect of procaine with 44°C 20-min treatment for Burkitt P3HR-1 cells showed a substantial enhancement of cytotoxicity in S phase cells. The probable implication of membrane composition, membrane changes due to heat and its modifying agents are discussed. Furthermore, when hyperthermia was combined with radiation in Burkitt P3HR-1 cells, cytotoxic enhancement was observed in G₁/S boundary phase, not in S phase.

Key Words:

• hyperthermia
• radiation
• cell-cycle
• procaine
• radiation sensitive mutant