Cyclosporine A-Loaded Solid Lipid Nanoparticles: Ocular Tolerance and In Vivo Drug Release in Rabbit Eyes

Abstract

Purpose: To determine the in vivo efficacy of cyclosporine A-loaded solid lipid nanoparticles (SLNs) in rabbit eyes.

Methods: SLNs were prepared and administered to the cul-de-sac of rabbits, and the drug amount in aqueous humor was detected by high performance liquid chromatography (HPLC). The irritation was evaluated by modified Draize testing.

Results: The particle size of SLNs was detected as 225.9 ± 5.5 nm with a negative surface charge. Aqueous humor drug levels reached 50.53 ng/mL, and there was no serious irritation in rabbit eyes.

Conclusions: Topical ophthalmic efficacy of cyclosporine A was enhanced via administration of SLNs.

KEYWORDS::

• Cyclosporine A
• In vivo release
• Modified Draize test
• Rabbit
• Solid lipid nanoparticles

ACKNOWLEDGMENTS

Declaration of interest: The authors report no conflict of interest. The authors alone are responsible for the content and writing of the paper.