Treatment With Triamcinolone Acetonide Prevents Decreased Retinal Levels of Decorin in a Rat Model of Oxygen-Induced Retinopathy

Abstract

Purpose: To investigate the effect of triamcinolone acetonide (TA) on retinal expression of decorin in a rat model of oxygen-induced retinopathy (OIR).

Materials and Methods: OIR was stimulated by exposing Sprague-Dawley (SD) rats to hyperoxia (80 ± 1.3% O₂) from postnatal day (P) 2 to P14 and then returning them to normoxia (room air, 21 ± 1.5% O₂). Control rats were maintained in normoxia. At P15, TA (40 mg/ml) was injected into the right vitreous of OIR rats and saline into the left vitreous of control rats. All rats were sacrificed at P18. RT-PCR, western blot and immunohistochemistry, TUNEL assay were performed to detect the effects of TA on molecular and morphological changes in retinal decorin levels in P18 OIR rats.

Results: In P18 OIR rats, mRNA and protein of retinal levels and immunoreactivity of retinal decorin were significantly less (p-value = 0.0000000012, 0.0007, 0.000003; n = 5; respectively) than in control rats. In addition, neuronal cell death was increased in P18 OIR rats (p-value = 0.0028; n = 5) relative to controls. However, treatment with TA prevented the decrease of mRNA, protein levels, and immunoreactivity in retinal decorin in P18 OIR rats (p-value = 0.00023, 0.003, 0.000079; n = 5, respectively), and restored neuronal cell death in P18 OIR rats (p-value = 0.0022, n = 5).

Conclusion: Our results suggest that decorin is involved in hypoxic retinal damage and that TA protects retinal neurons damaged by relative hypoxia from decreased decorin levels.

KEYWORDS::

• Decorin
• Ganglion cells
• Oxygen-induced retinopathy
• Retinal neurons
• Triamcinolone acetonide

ACKNOWLEDGMENTS

This work was supported by grant No. 2009-0068732 from the Basic Research Program of the Korea Science & Engineering Foundation. In addition, this study was partially supported by the BK21 Program and by the MRC program of MOST/KOSEF (R13-2005-012-01001-1).

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.