Structural Collagen Alterations in Macular Corneal Dystrophy Occur Mainly in the Posterior Stroma

Abstract

Purpose: Collagen fibrils in the corneal stroma in macular corneal dystrophy, on average, are more closely spaced than in the normal cornea. This study was conducted to investigate if this occurs uniformly across the stroma or is more prevalent at certain stromal depths.

Methods: Microbeam synchrotron X-ray fiber diffraction patterns were obtained in 25 μm steps across the whole thickness of a thin strip of a macular corneal dystrophy cornea obtained at keratoplasty. Data were analyzed for mean collagen interfibrillar spacing at all positions. Serum was analyzed immunochemically to determine immunophenotype, and transmission electron microscopy was carried out to visualize stromal ultrastructure.

Results: Keratan sulphate was not detectable in blood serum, classifying the disease as macular corneal dystrophy type I. Collagen interfibrillar spacing dropped linearly with stromal depth from the anterior to posterior cornea, measuring 5–10% less in the posterior 100 µm of the MCD stroma compared to the anterior 100 µm (p < 0.001). Isolated pockets of collagen fibrils with unusually large diameters were identified in the deep stroma.

Conclusions: Collagen fibril spacing is reduced and large-diameter collagen fibrils are seen in macular corneal dystrophy type I, with the deep stroma affected more. We speculate that the ultrastructural abnormalities are more prevalent in the posterior stroma because the structural influence of sulphated keratan sulphate glycosaminoglycans/proteoglycans is high in this region of the cornea.

KEYWORDS::

• Collagen
• Cornea
• Glycosaminoglycans
• Macular corneal dystrophy
• Proteoglycans

ACKNOWLEDGMENTS

Our research programs are funded by BBSRC Project Grant BB/D001919/1 (U.K.), NIH/NEI Research Grant EY014620 (U.S.A.), MRC Program Grant G0600755 (U.K.), EPSRC Project Grant EP/F034970/1 (U.K.), and beamtime access awards from The Japan Synchrotron Radiation Research Institute. B.P.P. is a graduate student sponsored by a BBSRC Doctoral Training Grant.

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.