Avastin Treatment Reduces Retinal Neovascularization in a Mouse Model of Retinopathy of Prematurity

Abstract

Purpose: This study was designed to evaluate the effect of one intraperitoneal (IP) injection of bevacizumab (Avastin) on the severity of oxygen-induced retinopathy (OIR) in a mouse model.

Materials and methods: Twenty-eight eyes of 14 mice with OIR were studied. There were nine mice in the bevacizumab-treated group (study group) and five mice in the saline-treated group (controls). The mouse OIR model consisted of a 5-day exposure to 75% oxygen. On postnatal day 12 (P12), Avastin 2.5 mg/kg was administered IP to the study group and 2.5 mg/kg normal saline was administered IP to the controls. All 14 mice underwent fluorescein angiography of the retinal vasculature on P17 and the following parameters were scored (Modified Retinopathy Scoring System, MRSS): blood vessel growth, formation of blood vessel tufts, extraretinal neovascularization, degree of central constriction, and tortuosity of vessels. In addition, the neovascular vessels were quantified on the hematoxylin and eosin (H&S)-stained paraffin sections of the eyes in a masked fashion.

Results: The MRSS score in the Avastin-treated mice was significantly lower than that of the saline-treated mice (3.06 ± 1.63 versus 7.1 ± 2.01, respectively, p = 0.0021). The neovascularization count was also significantly lower in the study group (3.44 ± 1.81 versus 9.34 ± 3.23 for the controls, p = 0.0013).

Conclusions: IP Avastin treatment reduced the extent of oxygen-induced retinopathy in a mouse model of retinopathy of prematurity.

Keywords::

• Avastin
• Mouse model
• Oxygen-induced Retinopathy
• Retinopathy of prematurity

ACKNOWLEDGMENT

Ronen Rabinowitz and Ayelet Priel have contributed equally to the work. This project was supported by a Maratier grant from the Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel.

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

NOTICE OF CORECTION

The version of this article published online ahead of print on 11 May 2012 contained errors on pages 627 and 628. Figure 3 and Figure 4 were switched. The corrected version is shown in this issue.