Intron F G79A Polymorphism of the Protein Z Gene in Turkish Behçet Patients

Abstract

Purpose: Vasculitis is one of the major findings of Behçet’s disease (BD). Protein Z (PZ) is a glycoprotein that acts as a cofactor of PZ-dependent protease inhibitor and suppresses trombus formation by inhibiting activated factor Xa. Polymorphism of the PZ gene was mentioned as a genetic risk factor for various thrombotic events. The aim of this study is to investigate the intron F G79A polymorphism of the PZ gene in Behçet patients with and without ocular involvement.

Methods: Seventy-six patients and 70 controls were included in the study. Intron F G79A polymorphism of PZ gene was determined by polymerase chain reaction based DNA analysis. The frequency of A allele and the distribution of genotypes were assessed by χ² test and the genotype distribution and Hardy–Weinberg equilibrium were tested with the χ² test for quality of fit.

Results: The frequency of the A allele was significantly higher in overall Behçet patients than in controls (odds ratio [OR] = 6.8; 95% CI, 2.6 to 17.9; p = 0.0001). It was also significantly higher in patients with (OR = 5.3; 95% CI, 1.83 to 15.6; p = 0.0024) or without (OR = 8.2; 95% CI, 2.95 to 22.5; p = 0.0001) ocular involvement compared to controls. However, A allele frequency was not significantly different between patients with eye involvement versus patients without eye involvement (OR = 0.65; 95% CI, 0.3 to 1.4; p = 0.28).

Conclusions: Although thrombosis in BD is multifactorial, intron F G79A polymorphism of PZ gene in BD may be one of the factors that contribute to this pathological process.

Keywords::

• Behçet’s disease
• Intron F G79A polymorphism
• Protein Z
• Thrombosis
• Vasculitis

Declaration of interest: The authors report no conflict of interest. The manuscript has not been published or submitted simultaneously for publication elsewhere. The study has been presented as a poster at the International Symposium on Uveitis, which took place in Constance, Germany, on 9–12 September 2008.