Ki-67 and bromodeoxyuridine labeling of human choroidal melanoma cells

Abstract

Understanding tumor growth patterns has implications for prognosis as well as for response and susceptiblity to treatment. The antibody Ki-67 was used as a marker of cycling cells and bromodeoxyuridine (BrdUrd) was used as a marker of proliferating cells to characterize the cycling and proliferative rates of cells from human choroidal melanoma. The BrdUrd labeling indices varied from 0–1.1% and the Ki-67 labeling indices ranged from 0–3.03%. Linear regression modeling showed good correlation defined by the equation: Ki-67 index −0.237 + 1.63 × BrdUrd labeling index with r = 0.919. Correlations between these indices and clinical and histologic parameters were not significant.