Cyclic AMP-dependent up-regulation of the taurine transporter in a human retinal pigment epithelial cell line

Abstract

This investigation was undertaken to study the role of cAMP in the regulation of the taurine transporter expressed in a human retinal pigment epithelial (HRPE) cell line. Treatment of the HRPE cells with cholera toxin for 24 h was found to stimulate the taurine transporter activity, as measured by taurine transport into the cells in the presence of NaCl, to a significant extent. The stimulation was 50–60% at 100 ng/ml cholera toxin. This stimulation was specific to the taurine transporter since the transport of two other amino acids (leucine and alanine), which are not substrates for the taurine transporter, was not affected by cholera toxin under similar conditions. Exposure of the cells to cholera toxin for a time period >4 h was needed to elicit the stimulatory effect. The cholera toxin-induced stimulation of the taurine transporter activity was associated with an increase in the maximal velocity of the transport system. The affinity of the transporter for taurine was not altered by the treatment. The stimulatory effect was markedly blunted when the treatment of the cells with cholera toxin was done in the presence of actinomycin D, an inhibitor of transcription, or cycloheximide, an inhibitor of translation. The increase in the taurine transporter activity induced by cholera toxin was associated with a 2.6-fold increase in the steady state levels of the transporter mRNA. Measurement of cyclic nucleotides in control and cholera toxin-treated cells revealed that the toxin caused a 20-fold increase in the cellular levels of cAMP, the levels of cGMP remaining unaffected. Treatment of the cells with membrane permeable cAMP analogs (dibutyryl cAMP and 8-bromo cAMP) or with agents which are known to increase intracellular cAMP levels (forskolin and isobutylmethylxanthine) also stimulated the taurine transporter activity. It is concluded that the taurine transporter expressed in the HRPE cells is subject to cAMP-dependent up-regulation and that the process involves de novo synthesis of the transporter protein.

Key Words:

• taurine transporter
• mRNA levels
• cyclic adenosine 3′,5′
• monophosphate (cAMP)
• cholera toxin
• regulation
• retinal pigment epithelium (RPE)
• human
• cell culture