Caffeic Acid Phenethyl Ester Suppresses the Induction of Eotaxin in Human Lung Fibroblast Cells

Abstract

Background. Eotaxin, a CC chemokine, plays an important role in inflammation of airway allergic diseases. The authors investigated the activities of caffeic acid phenethyl ester (CAPE), the active component of propolis, in regulating eotaxin production in human lung fibroblast. Material and Methods. The authors used human lung fibroblasts, CCD-11Lu cells, stimulated with interleukin-13 (IL-13) and tumor necrosis factor-alpha (TNF-α), to induce eotaxin secretion. The cells were treated with CAPE of different concentrations and pretreatment duration to check its inhibition in eotaxin production. Enzyme-linked immunosorbent assay (ELISA) was used to measure eotaxin secretion; electrophoretic mobility shift assay (EMSA) to check nuclear factor kappa B (NF-κB)–promoter binding; and Western blot to quantitate the cyplasmic inhibitor of NF-κB (IκB) and nuclear NF-κB p65. Results. CAPE inhibited the production of eotaxin in CCD-11Lu cells stimulated by IL-13 and TNF-α combination in a dose- and time-dependent manner. The authors also demonstrated CAPE to be able to inhibit NF-κB activation in CCD-11Lu cells. Conclusion. The authors suggest that CAPE is a promising agent in controlling eosinophils influx in human airway.

Keywords :

• caffeic acid phenethyl ester
• eotaxin
• IL-13
• lung fibroblasts
• TNF-α
• transcriptional factor