Abstract
The combination of superparamagnetic particles (SPP) as a ‘negative’ contrast agent for the liver parenchyma and Cr-HIDA as a ‘positive’ one for the bile ducts was tested in dogs. The maximum effect of SPP was present about 30 minutes after injection with a reduction of the image intensity of the liver close to the background noise level at the highest dosages. This effect lasted for about 4 to 5 hours and it had disappeared after 24 hours. Before any contrast administration or after Cr-HIDA the bile ducts were not discernible, but a high signal in the gallbladder was present 15 to 30 minutes after injection of Cr-HIDA. After SPP the wider bile ducts were discernible because of the lowering of the signal intensity in the liver. When SPP were followed by Cr-HIDA, the bile had a higher signal intensity, and even tiny bile ducts were visible. After cholecystokinin visualization of the choledochus duct was achieved as well as contrast filling of the duodenum. The blood, urine and liver function tests were found normal during the experiments. The combination of superparamagnetic particles and Cr-HIDA seems to be a promising method for MR-cholangiography. An evaluation of the anatomic structures of the liver should be possible with this method in different pathologies.