6
Views
4
CrossRef citations to date
0
Altmetric
Original Article

Histological and Histochemical Studies on Effects of Some Storage Solutions on Human Split Thickness Skin Grafts

, &
Pages 202-206 | Received 21 Feb 1974, Published online: 08 Jul 2009
 

Abstract

In order to improve short-term storing of skin grafts a new solution, Histocon, was tested. This solution has earlier been used as a transport medium for biopsies subjected to enzyme histochemical analyses. The capacities of Histocon, isotonic saline and a potassium-enriched Ringer solution to preserve the morphology, chemical components and enzyme proteins of the skin graft, were tested. All solutions were compared with fresh-frozen controls during the test period (2–21 days). With Histocon the morphology was identical with the fresh-frozen controls during the whole test period but both the other solutions produced increasingly marked changes after two days. A smaller loss of stainable chemical components was noted with Histocon than with the other solutions. The enzyme histochemical tests showed that Histocon did not visibly affect the hydrolytic enzyme activities while the other solutions rapidly decreased them. With Histocon, strong diaphorase activity was present for a longer period than that of the dehydrogenases.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.