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ABSTRACT
Purpose of the study: Follicular dendritic cell-secreted protein (FDC-SP) has been found to be expressed in periodontal ligament (PDL), a layer of soft connective tissue between tooth root and alveolar bone, and involved in immunoreaction. This study was performed to explore the potential role of FDC-SP in periodontal disease. Materials and Methods: The human periodontal ligament cells (hPDLCs) were stimulated with Porphyromonas gingivalis (P. gingivalis) lipopolysaccharide (LPS) and FDC-SP expression was examined by real-time PCR and western blot. Then this molecule was overexpressed or silenced in hPDLCs by transfection of FDC-SP expression plasmids or its small-interfering (si) RNA, respectively, and the effects of FDC-SP on expression of osteogenesis- and osteoclastogenesis-related genes in hPDLCs were analyzed by real-time PCR and western blot. Results: Our results showed that P. gingivalis LPS upregulated FDC-SP expression in hPDLCs. Overexpression of FDC-SP could decrease the expression of osteogenesis-related genes, increase the expression of osteoclastogenesis-related genes and RANKL/OPG ratio in hPDLCs. Meanwhile, silence of FDC-SP expression in hPDLCs remarkably inversed the above results. Conclusions: LPS-induced upregulation of FDC-SP expression in hPDLCs may enhance osteoclastogenesis in periodontal disease.
Acknowledgments
We are grateful to Professor Aaron J. Marshall and Dr Sen Hou (Department of Immunology, University of Manitoba, Winnipeg, Canada) for providing FDC-SP expression plasmid for this study.
Declaration of interest
No potential conflicts of interest were noted.