Abstract
Bovine articular cartilages from two sites within the lower femoral epiphysis were extracted with GuHCl and purified by CsCl density gradient centrifugation in two steps as described by Hascall and Sajdera1,2. Ninety per cent of the hexosamine containing material was extracted with 4 M GuHCl. The gel chromatographic properties of PGC and of PGS showed no or minor differences between the two sites. The amino acid, neutral sugar, uronic acid and hexosamine compositions were the same for the cartilage from the two sites.