The Glycosaminoglycan Constituents of Alveolar and Basal Bone of the Rabbit

Abstract

Proteoglycan was extracted from alveolar and basal bone of New Zealand White rabbits using a sequential extraction procedure. Proteoglycans not associated with the bone mineral represented 1% of the total organic matrix whereas proteoglycans associated with the mineral represented 20% of the alveolar organic matrix and 12% of the basal organic matrix. Chondroitin-4-sulphate and keratan sulphate were identified in both alveolar and basal bone following protease treatment of the proteoglycan extracts and enzymic digestion with chondroitinase AC, ABC and keratanase. Differences were observed in the percentage of each glycosaminoglycan (GAG) in the total organic matrix. In alveolar bone samples, keratan sulphate and chondroitin-4-sulphate is present in equal proportions. In basal bone chondroitin-4-sulphate represents approximately half the value found in alveolar bone and keratan sulphate about a quarter. The extracts were examined by gel filtration on Sepharose 4B under associative conditions. The 280 nm absorbance profiles of proteoglycan from alveolar and basal bone were essentially similar with three main peaks evident, including molecular weight material in excess of 2 × 10⁶. The bulk of the bone GAG appeared in the medium molecular weight range with trace amounts in lower molecular weight fractions.