An in-vitro Method of Investigating the Rates of Transcapillary Exchange and Net Filtration by Single-injection Technique

Abstract

A method of preparing a cat's hind leg that is suitable for studying the rates of transcapillary solute and solvent movements is described. The preparation may be perfused at any desired venous pressure. A bolus may be injected into the capillary network without altering either the perfusion pressure or the flow rate (while continuously recording the weight of the isolated leg). A new method of calculating extractions is introduced. This method uses the estimate of the correlation between the relative concentrations of reference and test substances in the venous outflow.