Trial and Error in Producing Ankylosing-Spondyhtis-Selective Antisera According to Andrew Geczy

C. J. Beukelman Eijkman-Winkler Laboratory of Medical Microbiology, Experimental Microbiology Section, Academic Hospital, Utrecht

H. C. Quarles Van Ufford Eijkman-Winkler Laboratory of Medical Microbiology, Experimental Microbiology Section, Academic Hospital, Utrecht

F. P. M. J. Van Bree Department of Immunohaematology and Bloodbank, Academic Hospital, Leiden, The Netherlands

P. C. Aerts Eijkman-Winkler Laboratory of Medical Microbiology, Experimental Microbiology Section, Academic Hospital, Utrecht

C. Nieuwenhoff Eijkman-Winkler Laboratory of Medical Microbiology, Experimental Microbiology Section, Academic Hospital, Utrecht

G. Reerink Eijkman-Winkler Laboratory of Medical Microbiology, Experimental Microbiology Section, Academic Hospital, Utrecht

A. Van Leeuwen Department of Immunohaematology and Bloodbank, Academic Hospital, Leiden, The Netherlands

H. Van Dijk Eijkman-Winkler Laboratory of Medical Microbiology, Experimental Microbiology Section, Academic Hospital, Utrecht

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Abstract

Geczy found that rabbit sera raised against Klebsiella strain K43 cross-reacted with the cells from HLA-B27 positive patients with ankylosing spondylitis (AS). Other laboratories failed to reproduce these results. After a series of unsuccessful attempts, however, we managed to prepare one selective antiserum, using E. coli, isolated from a Dutch Bechterew patient, in offspring of rabbits Geczy sent us. Ever since we obtained irreproducible results only. This paper reports about the many attempts we have made to produce a discriminating antiserum for use in a combined vital stain and dye-exclusion assay.

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Trial and Error in Producing Ankylosing-Spondyhtis-Selective Antisera According to Andrew Geczy

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Trial and Error in Producing Ankylosing-Spondyhtis-Selective Antisera According to Andrew Geczy

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