Development of a Solid Phase Enzyme Immunoassay for the Detection of Anti-Ro Autoantibodies

T. Dörner Department of Internal Medicine, Medical School (Charité), Humboldt University, Berlin, Germany

F. Hiepe Department of Internal Medicine, Medical School (Charité), Humboldt University, Berlin, Germany

F. Mielke Department of Internal Medicine, Medical School (Charité), Humboldt University, Berlin, Germany

S. T. Kiessig Department of Institute of Medical Immunology, Medical School (Charité), Humboldt University, Berlin, Germany

A. Lukowsky Department of Internal Medicine, Medical School (Charité), Humboldt University, Berlin, Germany

E. Apostoloff Department of Internal Medicine, Medical School (Charité), Humboldt University, Berlin, Germany

Abstract

An enzyme immunoassay was developed to detect anti-Ro(SS-A) autoantibodies. Both Ro-antigen components (52 and 60 kD) were purified from a pig spleen extract, using fast protein liquid chromatography (FPLC). Anti-La, anti-RNP, anti-DNA and anti-Sm antibodies do not react to the purified antigen. There was a strong correlation between anti-Ro activity in EIA and the titers in counter Immunoelectrophoresis (r_s= 0,893). Anti-Ro antibodies were found in 54 (69.2%) of 78 SLE sera by the developed EIA.

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Development of a Solid Phase Enzyme Immunoassay for the Detection of Anti-Ro Autoantibodies

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Development of a Solid Phase Enzyme Immunoassay for the Detection of Anti-Ro Autoantibodies

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