Automated 24-hours sampling of subcutaneous tissue free cortisol in humans

Abstract

Hormonal systems are major regulators of metabolic and cognitive function and many of these, including the critical stress-responsive hypothalamic-pituitary-adrenal (HPA) axis, release their constituent hormones in a circadian manner. This circadian rhythmicity is made up from an underlying approximate hourly ultradian rhythm. In order to understand the importance of both circadian and ultradian rhythms in man it is important to be able to carry out multiple sampling studies over extended periods in a subject’s home setting, which is the most meaningful physiological setting for homeostatically important hormones. This study has developed a novel automated sampling system that, when used in combination with a microdialysis system, collects timed samples of microdialysis fluid over a full 24 h in individuals going about their normal everyday activity. The apparatus has the capacity to provide sufficient sample volumes to measure changes in hormone concentration over 24 h, including the important period when subjects are asleep.

• Automated sampling
• circadian rhythm
• cortisol
• hypothalamic-pituitary-adrenal axis
• microdialysis

Acknowledgements

This work was carried out with financial support from the European Union – FP7 grant to OPTIMI. We should also like to thank Dr Suzanne Phillips for her help in the initial planning of this study. A patent application for this device has been filed.