Separation of Human Hemoglobins by Deae-Cellulose Chromatography using Glycine-Kcn-Nacl Developers

E. C. Abraham Laboratory of Protein Chemistry and Comprehensive Sickle Cell Center, Medical College of Georgia, and the V.A. Hospital, Augusta, Ga, 30902, USA

A. Reese Laboratory of Protein Chemistry and Comprehensive Sickle Cell Center, Medical College of Georgia, and the V.A. Hospital, Augusta, Ga, 30902, USA

M. Stallings Laboratory of Protein Chemistry and Comprehensive Sickle Cell Center, Medical College of Georgia, and the V.A. Hospital, Augusta, Ga, 30902, USA

T. H. J. Huisman Laboratory of Protein Chemistry and Comprehensive Sickle Cell Center, Medical College of Georgia, and the V.A. Hospital, Augusta, Ga, 30902, USA

Abstract

This chromatographic procedure uses DEAE-cellulose as ion exchanger and glycine-KCN-NaCl solutions as developers. Blood samples from several adults and newborn infants with α, β, δ, or γ chain variants have been analysed. The hemoglobins are eluted as compact and symmetrical zones, and the separation of many hemoglobin types is greatly improved. The procedure is relatively fast, simple, and inexpensive.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Separation of Human Hemoglobins by Deae-Cellulose Chromatography using Glycine-Kcn-Nacl Developers

Information for

Open access

Opportunities

Help and information

Separation of Human Hemoglobins by Deae-Cellulose Chromatography using Glycine-Kcn-Nacl Developers

Abstract

Reprints and Corporate Permissions

Academic Permissions

Related research

To cite this article:

Download citation

Information for

Open access

Opportunities

Help and information

Keep up to date

Your download is now in progress and you may close this window

Login or register to access this feature