An Improved Chromatographic Procedure for Quantitation of Human Fetal Hemoglobin

E. C. Abraham Laboratory of Protein Chemistry, Department of Cell and Molecular Biology and Comprehensive Sickle Cell Center, Medical College of Georgia, and Veterans Administration Hospital, Augusta, Georgia, 30901, USA

A. Reese Laboratory of Protein Chemistry, Department of Cell and Molecular Biology and Comprehensive Sickle Cell Center, Medical College of Georgia, and Veterans Administration Hospital, Augusta, Georgia, 30901, USA

M. Stallings Laboratory of Protein Chemistry, Department of Cell and Molecular Biology and Comprehensive Sickle Cell Center, Medical College of Georgia, and Veterans Administration Hospital, Augusta, Georgia, 30901, USA

F. A. Carver Laboratory of Protein Chemistry, Department of Cell and Molecular Biology and Comprehensive Sickle Cell Center, Medical College of Georgia, and Veterans Administration Hospital, Augusta, Georgia, 30901, USA

T. H. J. Huisman Laboratory of Protein Chemistry, Department of Cell and Molecular Biology and Comprehensive Sickle Cell Center, Medical College of Georgia, and Veterans Administration Hospital, Augusta, Georgia, 30901, USA

Abstract

The DW-cellulose chromatographic procedure which uses glycine-KCN-NaC1 solutions as Developers and a NaCl gradient to elute the hemoglobin zones was found to be useful for the quantitation of Hb F In samples containing Hb A, provided the level of Hb F exceeds 2%. The method has been evaluated through a study of artificial mixtures and of blood samples from patients with various disorders. The data have been compared with results obtained by four other procedures that are used for the quantitation of Hb F.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

An Improved Chromatographic Procedure for Quantitation of Human Fetal Hemoglobin

Information for

Open access

Opportunities

Help and information

An Improved Chromatographic Procedure for Quantitation of Human Fetal Hemoglobin

Abstract

Reprints and Corporate Permissions

Academic Permissions

Related research

To cite this article:

Download citation

Information for

Open access

Opportunities

Help and information

Keep up to date

Your download is now in progress and you may close this window

Login or register to access this feature