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Hemoglobin
international journal for hemoglobin research
Volume 3, 1979 - Issue 1
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Original Article

The Separation of Human Globin Chains by Ion-Exchange chromatography on Cm-Sepharose Cl-6B

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Pages 13-20 | Received 28 Jul 1978, Accepted 12 Dec 1978, Published online: 07 Jul 2009
 

Abstract

A chromatographic procedure for the separation of human globin chains is described. This method uses CM-Sepharose CL-6B as ion-exchanger and NaCl gradients formed in sodium phosphate buffers containing urea and 0.05M mercaptoethanol to elute the chains. One advantage of this system is that a column, once packed, may be used repeatedly for several chain separations, thus reducing cost and practical work. The resolution in our hands was also better than that on CM Cellulose using phosphate buffer gradients.

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