Oxygen Binding of Fetal and Adult Bovine Hemoglobin in the Presence of Organic Phosphates and Uric Acid Riboside

R. C. Smith Department of Animal and Dairy Sciences, Auburn University, Agricultural Experiment Station, Auburn, Alabama, 36830; (R.C.S. and G.J.G) and Research Laboratories of the Veterans Administration Hospital; Department of Medicine, University of Miami School of Medicine, Miami, Florida, 33125, (R.E.I, and D.R.H.)

G. J. Garbutt Department of Animal and Dairy Sciences, Auburn University, Agricultural Experiment Station, Auburn, Alabama, 36830; (R.C.S. and G.J.G) and Research Laboratories of the Veterans Administration Hospital; Department of Medicine, University of Miami School of Medicine, Miami, Florida, 33125, (R.E.I, and D.R.H.)

R. E. Isaacks Department of Animal and Dairy Sciences, Auburn University, Agricultural Experiment Station, Auburn, Alabama, 36830; (R.C.S. and G.J.G) and Research Laboratories of the Veterans Administration Hospital; Department of Medicine, University of Miami School of Medicine, Miami, Florida, 33125, (R.E.I, and D.R.H.)

D. R. Harkness Department of Animal and Dairy Sciences, Auburn University, Agricultural Experiment Station, Auburn, Alabama, 36830; (R.C.S. and G.J.G) and Research Laboratories of the Veterans Administration Hospital; Department of Medicine, University of Miami School of Medicine, Miami, Florida, 33125, (R.E.I, and D.R.H.)

Abstract

The P₅₀ values of “stripped” fetal and adult bovine hemoglobin were 18.4 and 23.9 respectively. Neither the oxygen-hemoglobin dissociation curve nor the Hill coefficient, n, of fetal or adult bovine hemoglobin was affected by uric acid riboside (UAR), 2,3-diphosphoglyceric acid (2,3-DPG), adenosine triphosphate (ATP), or inositol pentaphosphate (IPP). Combinations of UAR and ATP with adult bovine hemoglobin or 2,3-DPG and ATP with fetal hemoglobin also had no effect. It was concluded that neither adult nor fetal bovine red cells contained an identifiable compound which affects the binding of oxygen to hemoglobin.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Oxygen Binding of Fetal and Adult Bovine Hemoglobin in the Presence of Organic Phosphates and Uric Acid Riboside

Information for

Open access

Opportunities

Help and information

Oxygen Binding of Fetal and Adult Bovine Hemoglobin in the Presence of Organic Phosphates and Uric Acid Riboside

Abstract

Reprints and Corporate Permissions

Academic Permissions

Related research

To cite this article:

Download citation

Information for

Open access

Opportunities

Help and information

Keep up to date

Your download is now in progress and you may close this window

Login or register to access this feature