The Determination of the Percentages of G_γ and A_γ Chains in Human Fetal Hemoglobin by HPLC

Abstract

A newly developed HPLC procedure (1) was used to study the percentages of ^Gγ and ^Aγ chains in the Hb F from newborn and patients with sickle cell anemia. The method gives data comparable to those obtained with a more laborious chemical procedure. However, the presence of the ^Aγ^T chain, i.e., the ^Aγ chain with an Ile→Thr substitution at position 75, interferes with the determination of the ^Gγ to ^Aγ ratio because the ^aγ^t and ^Gγ chains have the same chromatographic mobility.