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Hemoglobin
international journal for hemoglobin research
Volume 5, 1981 - Issue 7-8
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Original Article

The Use of Globin Chain Electrophoresis in Polyacrylamide Gels for the Quantitation of the Gγ to Aγ Ratio in fetal Hemoglobih

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Pages 637-651 | Received 08 May 1981, Accepted 06 Oct 1981, Published online: 07 Jul 2009
 

Abstract

Polyacrylamide gel electrophoresis (PAGE) in the presence of urea, acid, and Triton X-100 was used for determination of the Gγ to Aγ ratio in human Hb F. The data compared most favourably with results obtained by a HPLC procedure and by a chemical procedure. Moreover, its accuracy and reproducibility was determined. The presence of Hb A2 in a sample with Hb F level below 10% interferes with the determination because of the nearly identical electro-phoretic mobilities of the δ and Gγ chains. Thus, the removal of Hb A2 is required. Samples free of Hb A2 but with a Hb F level as low as 2% can be analyzed with an accuracy of about 5%. The PAGE method has been applied to the Hb F of 63 subjects with β thalassemia and related conditions, and the results of these analyses are included in this communication.

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