Evidence for Multiple Differing Cation Binding Sites on Hemoglobin A and S

G. Amiconi CNR Center of Molecular Biology, Institutes of Chemistry and Biochemistry, Faculty of Medicine, University of Rome, Rome; Laboratory of Molecular Biology, University of Came-rino, Caraerino, Italy

L. Civalleri CNR Center of Molecular Biology, Institutes of Chemistry and Biochemistry, Faculty of Medicine, University of Rome, Rome; Laboratory of Molecular Biology, University of Came-rino, Caraerino, Italy

S. G. Condõ CNR Center of Molecular Biology, Institutes of Chemistry and Biochemistry, Faculty of Medicine, University of Rome, Rome; Laboratory of Molecular Biology, University of Came-rino, Caraerino, Italy

F. Ascoli CNR Center of Molecular Biology, Institutes of Chemistry and Biochemistry, Faculty of Medicine, University of Rome, Rome; Laboratory of Molecular Biology, University of Came-rino, Caraerino, Italy

R. Santucci CNR Center of Molecular Biology, Institutes of Chemistry and Biochemistry, Faculty of Medicine, University of Rome, Rome; Laboratory of Molecular Biology, University of Came-rino, Caraerino, Italy

E. Antonini CNR Center of Molecular Biology, Institutes of Chemistry and Biochemistry, Faculty of Medicine, University of Rome, Rome; Laboratory of Molecular Biology, University of Came-rino, Caraerino, Italy

Abstract

Some divalent cations (at 10⁻³M concentration) have been shown to act as oxygen-linked ligands. The effect of Ca⁺⁺ (only on hemoglobin S) and Zn⁺⁺ (on normal hemoglobin and hemoglobin S) can be traced to a preferential binding of these cations to the oxy- conformation of the proteins; the reverse is true for Ni⁺⁺, since its presence reduces the oxygen affinity of both hemoglobins. The results suggest: i) the existence of at least two different cation binding sites on hemoglobin; ii) the replacement of glutamic acid by valine in hemoglobins S introduces sufficient structural modification to form a new cation binding site.

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Evidence for Multiple Differing Cation Binding Sites on Hemoglobin A and S

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Evidence for Multiple Differing Cation Binding Sites on Hemoglobin A and S

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