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Hemoglobin
international journal for hemoglobin research
Volume 6, 1982 - Issue 2
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Original Article

Characterization and Quantitation of Glycosylated Hemoglobins in Canine Blood

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Pages 143-151 | Received 12 Nov 1981, Accepted 26 Feb 1982, Published online: 07 Jul 2009
 

Abstract

Canine hemolysates exhibited two minor and one major hemoglobins when chromatographed on Bio Rex-70 column with 0.05 M phosphate-0.01 M KCN (pH 6.5) containing 0.015 M NaCl. the first minor fraction was electrophoretically heterogeneous, constituting at least two hemoglobins. the second was completely homogeneous with somewhat faster anodal mobility than the major one and the difference was shown to be localized solely in the B subunits. Thiobarbituric acid reaction was strongly positive for this hemoglobin, indicating the presence of glycosyl ketoamine linkage. We describe a simple and reliable procedure for quantitation of the canine glycosylated hemoglobins with a home-made Bio Rex-70 mini-column.

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