Preparation and Properties of Nickel Hemoglobin

Kenneth Alstont Laboratory of Chemical Biology National Institute of Arthritis, Diabetes, and Digestive and Kidney Diseases National Institutes of Health, Bethesda, Maryland, 20205; Department of Biological Sciences, Columbia University New York, New York, 10027; Division of Health & Natural Sciences, Benedict College, Columbia, SC, 29204

Alan N. Schechter Laboratory of Chemical Biology National Institute of Arthritis, Diabetes, and Digestive and Kidney Diseases National Institutes of Health, Bethesda, Maryland, 20205; Department of Biological Sciences, Columbia University New York, New York, 10027

John P. Arcoleo Laboratory of Chemical Biology National Institute of Arthritis, Diabetes, and Digestive and Kidney Diseases National Institutes of Health, Bethesda, Maryland, 20205; Department of Biological Sciences, Columbia University New York, New York, 10027; Institute of Cancer Research, Columbia University, New York, NY, 10032

Jonathan Greer Laboratory of Chemical Biology National Institute of Arthritis, Diabetes, and Digestive and Kidney Diseases National Institutes of Health, Bethesda, Maryland, 20205; Department of Biological Sciences, Columbia University New York, New York, 10027; Abbott Laboratories, Abbott Park, North Chicago, IL, 60064

Gary R. Parr Laboratory of Chemical Biology National Institute of Arthritis, Diabetes, and Digestive and Kidney Diseases National Institutes of Health, Bethesda, Maryland, 20205; Department of Biological Sciences, Columbia University New York, New York, 10027; Department of Biochemistry, Clemson University, Clemson, SC, 29631

Fred K. Friedman Laboratory of Chemical Biology National Institute of Arthritis, Diabetes, and Digestive and Kidney Diseases National Institutes of Health, Bethesda, Maryland, 20205; Department of Biological Sciences, Columbia University New York, New York, 10027; Laboratory of Molecular Carcinogenesis, National Cancer Institute, Bethesda, MD, 20205

Abstract

Hemoglobin A reconstituted with nickel protoporphyrin IX (NiHbA) has been prepared and characterized. Kinetics of its reaction with p-mercuribenzoate and with haptoglobin, absorption and circular dichroism spectra, and x-ray crystallographic properties have been investigated as probes of its structural conformation. The results suggest that NiHbA exists in a structure that is similar to the deoxy, or T-state of HbA. It is proposed that NiHbA and its derivatives may serve as a useful model for future studies of hemoglobin allosteric changes.

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Preparation and Properties of Nickel Hemoglobin

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Preparation and Properties of Nickel Hemoglobin

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