Hb F-Tokyo or α₂GΓ₂34(B16) Val→Ile, A Silent Γ Chain Variant Detected by Reverse Phase high Performance Liquid Chromatography

S. S. Chen Laboratory of Protein Chemistry Department of Cell and Molecular Biology, Medical College of Georgia, Augusta, GA, 30912, USA

J. B. Wilson Laboratory of Protein Chemistry Department of Cell and Molecular Biology, Medical College of Georgia, Augusta, GA, 30912, USA

B. B. Webber Laboratory of Protein Chemistry Department of Cell and Molecular Biology, Medical College of Georgia, Augusta, GA, 30912, USA

T. H. J. Huisman Laboratory of Protein Chemistry Department of Cell and Molecular Biology, Medical College of Georgia, Augusta, GA, 30912, USA

S. Miwa Institute of Medical Science University of Tokyo, Tokyo, Japan

Y. Amenomori Department of Obstetrics Japan Red, Cross Medical Center, Tokyo, Japan

Abstract

Hb F-Tokyo with a Valine→Isoleucine replacement at position γ34(B16) is a Gγ chain variant which was discovered by reverse phase chromatography as this method permitted the nearly complete separation of the three types of γ chain. The chemical characterization was greatly facilitated by the use of a larger, preparative, HPLC column which allowed the isolation of sufficient quantities of the different γ chains.

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Hb F-Tokyo or α₂GΓ₂34(B16) Val→Ile, A Silent Γ Chain Variant Detected by Reverse Phase high Performance Liquid Chromatography

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Hb F-Tokyo or α2GΓ234(B16) Val→Ile, A Silent Γ Chain Variant Detected by Reverse Phase high Performance Liquid Chromatography

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Hb F-Tokyo or α₂GΓ₂34(B16) Val→Ile, A Silent Γ Chain Variant Detected by Reverse Phase high Performance Liquid Chromatography