The estimation of Hb A₂ IN the presence of Hb C or Hb E by reverse phase high performance liquid chromatography

W. A. Schroeder Division of Chemistry and Chemical Engineering, California Institute of Technology Pasadena, CA, 91125

Joan B. Shelton Division of Chemistry and Chemical Engineering, California Institute of Technology Pasadena, CA, 91125

J. Roger Shelton Division of Chemistry and Chemical Engineering, California Institute of Technology Pasadena, CA, 91125

Van Huynh Division of Chemistry and Chemical Engineering, California Institute of Technology Pasadena, CA, 91125

Abstract

Although Hb-C may be separated from Hb A₂ by some ion exchange methods, most will not separate Hb E and Hb A₂. The δ chain can be readily separated from the β^c, β^E and β^O–Arab chains by reverse phase HPLC. Hence, reverse phase HPLC provides a means of quantitatively determining Hb A₂ in the presence of Hb C, Hb E, and Hb O-Arab. The procedure, although not highly accurate, does permit the detection of increased Hb A₂, for example, in β-that heterozygotes and, therefore, is applicable to other conditions (Hb C, Hb E, Hb O-Arab).

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The estimation of Hb A₂ IN the presence of Hb C or Hb E by reverse phase high performance liquid chromatography

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The estimation of Hb A₂ IN the presence of Hb C or Hb E by reverse phase high performance liquid chromatography