Ultrathin-layer polyacrylamide gel isoelectric focusing for the identification of hemoglobin variants: Hemoglobin: Vol 10, No 2

Hemoglobin

international journal for hemoglobin research

Volume 10, 1986 - Issue 2

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Abstract

An ultrathin-layer polyacrylamide gel isoelectric focusing method for typing hemoglobin is described. This method, which uses Pharmalyte pH 6.7-7.7 and an electrode wick distance of 5.0 cm, clearly resolves the A, F, S, C and a number of rare variant allelic products. The technique is ideal for rapid screening of a large number of fresh blood and/or bloodstained samples.

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Ultrathin-layer polyacrylamide gel isoelectric focusing for the identification of hemoglobin variants

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Ultrathin-layer polyacrylamide gel isoelectric focusing for the identification of hemoglobin variants

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